Aureobasidin A (9CI)
Aureobasidin A,AbA
CAS: 127785-64-2
Molecular Formula: C60H92N8O11
Aureobasidin A (9CI) - Names and Identifiers
Aureobasidin A (9CI) - Physico-chemical Properties
| Molecular Formula | C60H92N8O11 |
| Molar Mass | 1101.42 |
| Density | 1.19±0.1 g/cm3(Predicted) |
| Boling Point | 1229.1±65.0 °C(Predicted) |
| pKa | 13.35±0.70(Predicted) |
Aureobasidin A (9CI) - Reference
| Reference Show more | Note: part of our products can only provide part of the information, our company does not guarantee the authority of the information provided, only for the customer reference Exchange research. storage conditions: 2-8 °c Note 1) for your safety and health, please wear an experimental suit and wear disposable gloves. 2) the optimal working concentration of AbA varies with different host cells and can be determined according to the minimum inhibitory concentration (MIC). 15) positive transformants were picked and/or the transformation efficiency (expressed as the number of colonies transformed per microgram of plasmid DNA) was measured. 14) inoculate a 100 μl cell suspension on YPD selection medium plates (containing a certain concentration of AbA, depending on the strain type). Transformation was completed after 3-4 days of incubation at 30 °c. 13) centrifuge at 5,000-10,000 rpm and suspend the pellet with 1-10 ml of 0.9% NaCl. 12) culture at 30 °c for 6 hours to overnight. 11) centrifuge at 5,000 rpm for 1 min and suspend the pellet with 5 ml of YPD medium. 10) place at room temperature for 10 minutes. 9) incubation at 30 °c for 30 minutes, followed by incubation at 42 °c for 15 minutes. 8) add 850 µL Solution B (formula: dissolve 40 g polyethene Glycol 4000 in 100 Solution A to be fully dissolved, need to be ready for use) and mix gently. [note]: pAUR101 needs to be transformed with linear DNA. The use of circular DNA may reduce the efficiency of the transformation or even be unsuccessful. pAUR112 and pAUR123 were transformed using intact plasmid DNA. 7) 5 μg vector (circular or linear DNA) and 150 μg Carrier DNA (which has been heated at 100 °c for 10 minutes and rapidly cooled) are added. 6) a 100 μl cell suspension was collected in a tube and cultured at 30 ° C. For 1 hour. 5) resuspend the pellet with Solution A until the OD660 was 150. 4) the pellet was suspended in 10 ml of Solution A (formula: 100 mM Lithium acetate,10 mM Tris-HCl pH 7.5,1 mM EDTA) and centrifuged at 1,000 x g for 5 minutes. 3) centrifuge at 1,000 x g for 5 min. 2) after incubation at 30 ° C. For about 6 hours, the OD660 was measured to be 1 to 2. When a diploid was used, the OD660 was determined to be 2 to 4. 1) add 0.5 ml of yeast cultured overnight to 50 ml of YPD medium (formula: 1L liquid medium containing 10g of East extract,20g of polypeptone, 20g of D-glucose; additional 2% Agar was added to the solid medium;). Procedure (AbA-Resistant Yeast transformation system; For reference only) |
Aureobasidin A (9CI) - Reference Information
| brief introduction | hexifenjing is a cyclic acetate antibiotic isolated from filamentous fungus (Aureobasidiumpullulans)Rioe and has strong antifungal ability. At a lower concentration (0.1-0.5 μg/ml), it can be toxic to yeast. Fungal species sensitive to it include: teething yeast (Saccharomyces cerevi Siae), Schizosaccharomyces pombe (Schizosaccharomycespombe), Candida glabrata (Candida glabrata), Aspergillus nidulans (Aspe;rgiIlusnidulans) and Aspergillus niger (A.niger.). |
| Mechanism of action | The mechanism of action is that hexifenjing inhibits the activity of inositol (inositol phospkxryIceramide,IPC) synthetase, which is dependent on fungal growth, Interfering with the synthesis of pin fat, thereby further killing the strain. Also, hexifengin does not disrupt DNA,RNA, and protein synthesis. |
| Preparation | The polyethylene glycol modified Basilifenjing has a strong dissolving ability, and due to the barrier formed by polyethylene glycol around Basilifenjing, the steric hindrance of macromolecules is produced, thereby reducing the enzymatic hydrolysis of Basilifenjing in the body, prolonging its half-life, and avoiding the rapid metabolic elimination of Basilifenjing in the kidney, improve its therapeutic effect. The method for preparing mPEG-C(= O)-Basafenjing is as follows: 1. mPEG-SC the preparation of the sample, polyethylene glycol PEG with different molecular weights are dissolved in an appropriate amount of dichloromethane respectively, 1.1 equivalent of DSC (disuccinimide carbonate),2 equivalent of DIEA (diisopropylethylamine), 0.2 equivalent of DMAP(N,N-dimethylaminopyridine) are added, temperature control is 60 ℃, reaction is 8h, concentration is added, crystallization of isopropyl ether to obtain PEG succinimide activation product PEG-SC with different molecular weights. The preparation of the polyethylene glycol modified basilfenjing of the present invention by mPEG-C a (= O)-basilfenjing sample comprises the following steps: adding a phosphate buffer solution to a basilfenjing solution, adjusting its pH to 6.0-9.0, and then adding a mPEG-SC to react at 5-40°C, preferably 25°C, for a reaction time of 2-6 hours, preferably 4 hours. The reaction mixture is separated by chromatography, the mobile phase is sodium chloride-containing acetic acid buffer, after chromatographic separation, concentration, freeze-drying to obtain different molecular weights of polyethylene glycol modified basilfenjing; the weight of basilfenjing solution is 1 mg/mL of aqueous solution. The polyethylene glycol modified Basifenjing can prepare antifungal drugs. |
Last Update：2024-04-09 02:00:08
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View History
Aureobasidin A (9CI)
富马酸替诺福韦酯杂质4
2-AMINO-6-FLUORO-3-FORMYLCHROMONE
2-四氢吡喃-3-亚基乙腈
L-Argininamide dihydrochloride
阿齐沙坦杂质49
2-[(4-异丙苯基)氨基]吡啶-3-羧酸
Copper(II) oxalate hemihydrate
N-环丁基-吡唑-4-频哪醇二硼酸酯
2-ethyl-1-hexanophosphate
富马酸替诺福韦酯杂质4
2-AMINO-6-FLUORO-3-FORMYLCHROMONE
2-四氢吡喃-3-亚基乙腈
L-Argininamide dihydrochloride
阿齐沙坦杂质49
2-[(4-异丙苯基)氨基]吡啶-3-羧酸
Copper(II) oxalate hemihydrate
N-环丁基-吡唑-4-频哪醇二硼酸酯
2-ethyl-1-hexanophosphate